bioGenous™ Mouse Intestinal Organoid Kit is a serum-free culture medium designed for mouse intestinal organoids derived from adult intestinal stem cells. Organoids grown in this complete medium predominantly consist of intestine stem cells (LGR5+), rapidly proliferating cells (KI67+), absorptive enterocytes (ALPi+), Paneth cells (ATOH1+), and goblet cells (MUC2+). These mouse intestinal organoids faithfully recapitulate the characteristics of in vivo intestinal epithelium in terms of self-renewal capacity, tissue architecture, cell type composition, and functionality. Therefore, they serve as an ideal in vitro model for studying intestinal homeostasis and disease mechanisms.