ATP, as a crucial energy molecule, reflects the metabolic level of living cells. Under both 2D and organoid culture conditions, ATP content shows a strong linear relationship with the number of living cells. Therefore, ATP quantification can be used to assess the number of living cells in organoids. bioGenous^TM Organoid Viability ATP Assay Kit is specially designed for microtissue detection in organoids, based on 2D cell viability assay reagents. bioGenous^TM Organoid Viability ATP Assay Kit is widely applicable for cell viability assays in various organoid samples, such as organoids and 3D tumor spheroids. Testing has shown that this product has enhanced lysis capability when used for cell viability detection in organoid cultures. For 3D microtissues cultured in Matrigel, it allows direct detection without the need to remove the Matrigel.

bioGenous^TM Organoid Viability ATP Assay Kit contains recombinant luciferase and luciferin, which can be directly added to the culture system for detection. The assay reagent automatically lyses the cells, releasing ATP from viable cells into the system, where it reacts with luciferase. The resulting chemiluminescence is proportional to the number of living cells in the system.