3D-Cell Clone Selection System is an automated device designed for selecting 3D cell and organoid clones. It enables precise picking of monoclonal 3D cells and organoids for subsequent standardized analysis or cultivation. This system has broad applications, including organoid library construction, monoclonal culture of gene-edited organoids, isolation of normal/diseased tissue-derived organoids, drug testing, and single-cell-level research. It drives the rapid advancement of organoid technology in foundational scientific research, drug discovery, precision medicine, and regenerative medicine.

3D-Cell Clone Selection System

Product Introduction:

• 3D-Cell Clone Selection System utilizes an automated workflow to precisely select 3D cells and organoids. The standard workflow can be divided into four key steps:

• The system features a user-friendly, multifunctional operation interface. The software automatically saves real-time, high-quality microscopic images before and after selection, providing researchers with a convenient experience for data storage and analysis.

Core Advantages of the Product:

• Standardization: Compatible with the selection of various normal tissue and tumor organoids.

• High Efficiency: Equipped with a dedicated organoid imaging and tracking system, enabling faster screening of more clones.

• Automation: Utilizes a high-precision three-axis robotic arm system for accurate positioning and selection of organoids.

• Ease of Operation: Supports real-time image preview, making the selection process quick and convenient.

• High Accuracy: Employs three-dimensional spatial positioning to accurately select target organoids, avoiding limited dilution.

• Flexibility: Supports both single and multiple clone selections, enhancing experimental flexibility.

Application Scenarios:

The system is widely applicable in the construction of organoid lines, clone purification of organoids, sorting of gene-edited organoids, rescue of primary contaminated organoids, tumor drug development, and single-cell-level research. It can rapidly select various types of tumor and normal tissue organoids, helping to quickly screen organoids that meet requirements and improve the success rate of organoid line establishment. This accelerates the advancement of organoid technology in scientific research, drug development, regenerative medicine, and precision medicine.

Application Case:

Standardized organoid lines are crucial for organoid research and obtaining reliable and stable experimental results. Using the 3D Cell Clone Selection System to select organoids in good growth conditions provides more reliable experimental assurance for drug testing and organoid line construction. Figure A shows microscopic images of human small intestine organoids and human trachea organoids selected using this device. Subsequently, the selected organoids were individually digested for standardized cultivation, with the cultivation results shown in Figure B, demonstrating the reliability and efficiency of the 3D Cell Clone Selection System.

The precise selection and standardized cultivation of human intestinal organoids

The precise selection and standardized cultivation of human tracheal organoids

The 3D Cell Clone Selection System provide researchers with an efficient and accurate clone screening solution. This system offers robust support for the advancement of organoid technology, driving life science research to new heights.