Basement membranes are thin continuous layers of specialized extracellular matrices that form the interface on which epithelial, endothelial, or neuronal cells grow. Basement membranes also support muscle and Schwann cells of the peripheral nerves as they are found surrounding these cells. The bioGenousTM Organoid Culture ECM is a soluble form of basement membrane extracted and purified from the Engelbreth-Holm-Swarm (EHS) tumor. The major constituents of the bioGenousTM Organoid Culture ECM include laminin, collagen IV, entactin/nidogen, and heparin sulfate proteoglycan.
bioGenousTM Organoid Culture ECM is a reduced growth factor hydrogel that allows the complete control of the organoid culture microenvironment to allow for more consistent cell growth and reproducible experiments. It supports the growth and maintenance of human embryonic stem cells (hESCs) and tissue-derived stem cells, and for the in vivo study of both healthy and tumor organoids derived from various organs. bioGenousTM Organoid Culture ECM is compatible with all culture media and gels at temperatures above 10°C to form a reconstituted basement membrane.