Organoid High-Throughput Bioprinting System
The Organoid High-Throughput Bioprinting System, independently developed by bioGenous, is an automated platform designed for standardized organoid plating and construction. This system is suitable for a wide range of applications, including the construction of organoid biobanks and high-throughput drug screening. It facilitates high-precision, high-throughput plating of organoids derived from various tumour and normal tissues, thereby accelerating the advancement of organoid-based technologies in drug discovery and precision medicine.
Organoid Bioprinter
Features and Benefits：
High Throughput: Equipped with single-channel and eight-channel printing modules to enable high-throughput and rapid organoid printing.
Standardization: Compatible with various types of extracellular matrices, supporting organoid printing across multiple well plate formats of different sizes and specifications.
Automation: Features a high-precision three-axis motion pipetting system for organoid mixing, dispensing, and printing.
Ease of Use: Supports preset programmes with customizable experimental protocols, allowing for quick and convenient workflow adjustments to expedite experimental processes.
Low-Temperature Operation: Equipped with multiple temperature control modules with customizable and interchangeable molds to accommodate a wide range of consumables.
Learn More About the Organoid Bioprinte:
l The experimental protocol of the Organoid Bioprinter is flexible and easily adjustable. It is simple to operate; just open the sample cover and place it into the pre-cooled sample tray to start the experiment
l Capable of rapid, precise, and flexible printing of organoids and extracellular matrix hydrogels.
The three-axis printing system,1+8 channels enabling high-throughput printing of organoids,
l Suitable for rapid and flexible high-throughput printing of organoids in various specifications of cell culture plates.
Array Printing:6-Well Plates: Each well supports up to 9 gel spots, with a printing volume of 10-12.5 µL per spot.
12-Well Plates: Each well supports up to 4 gel spots, with a printing volume of 10-12.5 µL per spot.mL。
Single-Spot Printing:24-Well Plates: Each well has a printing volume of 25-35 µL.mL，48-Well Plates: Each well has a printing volume of 10-15 µL.mL，96-Well Plates: Each well has a printing volume of 3-5 µL.mL.
Case Presentation
Printing of 293T Cells and Lung Adenocarcinoma Organoids
The Organoid Bioprinter was employed by bioGenous customers to print 293T cells and lung adenocarcinoma organoids, and the coefficient of variation (CV) was measured by the bioGenous LivingCell-FluoTM Organoid Vitality Assay Kit (#E238004) to evaluate the consistency and precision of the printing. The results indicated that the Organoid Bioprinter delivers significantly superior performance compared to manual printing in terms of consistency and accuracy.
Data sourced from customer laboratories
Assessment of Growth Consistency for Colorectal Cancer Organoids Printed by the Organoid Bioprinter
The identical colorectal cancer organoid samples were uniformly distributed into four wells via the Organoid Bioprinter, and the growth of organoids in these wells was monitored over three days using the bioGenous LivingCell-FluoTM Organoid Vitality Assay Kit (#E238004). By comparing the growth curves, it was demonstrated that the growth curves for organoids in all four wells were consistent, indicating that the Organoid Bioprinter provides excellent printing consistency
Precise Plating and High-Throughput Drug Screening for Colorectal Cancer Organoids
The accurate and stable printing of organoids is crucial for obtaining reliable and consistent experimental results. The high-throughput organoid bioprinting system provides superior plating precision compared to manual methods, ensuring accurate and reliable experimental results for drug testing. Using this system, colorectal cancer organoids were printed in a 96-well plate and cultured for 6 days (growth process shown in Figure B). Subsequently, three drug screening experiments were conducted, with the drug sensitivity curves showed in Figure D, demonstrating stable and consistent drug sensitivity results
(A) High-Throughput Precise Plating of Colorectal Cancer Organoids. (B) Growth Process of Colorectal Cancer Organoids. (C) State of Colorectal Cancer Organoids After Drug Treatment. (D) Drug Sensitivity Curves for Colorectal Cancer Organoids.
Standardized Organoid Printing to Support Biobank Development
Organoid Bioprinter Ordering Information
| Cat.No. | Description | Qty/Cs |
| Organoid Bioprinter | Organoid Bioprinter | 1 |
| BP241031 | bioprinting pipette tip A (with filter) | 50 |
| BP241032 | bioprinting pipette tip B(with filter) | 50 |
