Unit 01-02 & 09-12, 8th Floor, Building 22, No. 388 Xinping Street, Suzhou Industrial Park, Suzhou Area, Jiangsu Pilot Free Trade Zone, China
Genetically Modified Organoid Models
Leveraging advanced gene editing technologies, we provide customized construction of precise gene-mutated organoid models. Our comprehensive solutions cover the whole process, including organoid construction, target design, carrier construction, viral packaging, infection efficiency analysis, positive clone screening, and expression level detection. These models enable in-depth analysis of the impact of gene mutations on disease occurrence and progression, supporting personalized medical research.
Hepatoblastoma Tumorigenesis Model: By overexpressing YAP15SA in human fetal liver organoids using a lentiviral vector, we have successfully constructed a single-gene-driven human hepatoblastoma tumorigenesis model. The genetically modified organoids were transplanted under the liver capsule of NSG immunodeficient mice, resulting in the development of hepatoblastoma and spontaneous lung metastases.
Gene Knockout in Mouse Intrahepatic Bile Duct Organoids: By transfecting mCherry-labeled gRNA into Cas9-overexpressing mouse intrahepatic bile duct organoids using a lentiviral vector, followed by flow cytometry sorting of double-positive organoids, we successfully generated bile duct organoids with targeted gene knockout. Sequencing can be further utilized to analyze the knocked-out gene in the organoids and the subsequent changes in other genes resulting from the knockout.
Effect pictures of infection/transfection of different organoids
