bioGenousTM Human Liver Ductal Organoid Kit is a chemically defined cell culture medium for establishment and maintenance of human liver ductal organoids(hLDs) derived from introhepatic cholangiocyte. Self-renewal of the ductal epithelium is driven by the proliferation of stem cells and their progenitors located in liver. hLDs display all hallmarks of the ductal epithelium in terms of architecture, cell type composition, and self-renewal dynamics, therefore hold great promise for unprecedented studies of human liver development and disease, hLDs may also have applications in regenerative biology through ex vivo expansion of the ductal epithelium.