Foreword
bioGenous is dedicated to providing premium organoid research products and solutions, empowering scientific research to reach new heights. We proudly introduce our new N-2 Serum-Free Supplement, a serum-free formulation developed based on the classic Bottenstein's N-1 formulation, specifically engineered to promote the growth of organoids derived from neural and epithelial cells.
Product Features
1. Chemically Defined, Serum-Free Formula
N-2 Serum-Free Supplement utilizes chemically defined supplements, eliminating uncertainties associated with animal serum to ensure stable and reliable experimental outcomes.
2. Highly Concentrated 100x Formula
Each 10mL bottle of N-2 Serum-Free Supplement is a 100-fold concentrated solution, requiring only minimal addition to meet extensive experimental demands—cost-effective and user-friendly. The highly concentrated formulation not only simplifies experimental preparation but also minimizes storage space requirements.
3. Broad Applicability
N-2 Serum-Free Supplement is compatible with various basal media, including those for neural cell or organoid culture. It can be used in combination with basal fibroblast growth factor (bFGF) and epidermal growth factor (EGF) to further enhance cell culture outcomes.
Premium Raw Materials and Rigorous Quality Control
To ensure the high quality and consistency of N-2 Serum-Free Supplement, we maintain rigorous standards throughout raw material selection and production. All ingredients undergo strict screening and testing to guarantee purity and biocompatibility. Every stage of the manufacturing process is subject to meticulous quality control, ensuring the reliability and stability of the final product.
We also employ advanced production technologies and equipment to ensure optimal composition ratios and performance in every batch.
Application Areas
N-2 Serum-Free Supplement is an ideal choice for neuroscience research, particularly suited for in vitro culture and study of neuroblastoma and mature neurons. It facilitates the analysis of neural system development, function, and pathological mechanisms. By optimizing cell culture conditions, researchers can more accurately observe and analyze neuronal behavior and interactions, driving innovation and progress in neuroscience.
N-2 Serum-Free Supplement is rich in serum-replacement nutrients essential for cell growth and differentiation. These components synergistically optimize organoid culture conditions, promoting proliferation, differentiation, and functional maturation of diverse cell types within organoids.
- Drug Screening and Toxicology Studies
During drug development, N-2 Serum-Free Supplement supports the culture and testing of neural cells and other cell types to evaluate the effects of novel compounds on the nervous system and other systems. By providing a stable and efficient cell culture environment, this supplement helps researchers efficiently screen for promising drug molecules while identifying potential toxicological risks, ensuring the safety and efficacy of new pharmaceuticals.
- Regenerative Medicine and Cell Therapy
N-2 serum-free supplement provides essential nutritional support for the in vitro expansion and differentiation of stem cells and neural stem cells, advancing research and applications in regenerative medicine and cell therapy. In stem cell research, it significantly enhances stem cell survival and proliferation rates, establishing a robust foundation for both basic and clinical translational studies related to neural cells.
Usage Instructions
It is recommended to add N-2 Serum-Free Supplement to the base medium, such as neural cell or organoid culture base medium, at a ratio of 1:100. Depending on specific experimental requirements, growth factors like bFGF and EGF may be added to optimize cell growth and functional expression. The N-2 Serum-Free Supplement offers simple and flexible usage, allowing researchers to adjust according to experimental needs to ensure optimal culture results.
Storage Conditions
To maintain the activity of N-2 Serum-Free Supplement, store it in a frozen environment at -20°C or -80°C, protected from light exposure. The product maintains stability for 18 months under frozen storage conditions, ensuring long-term reliability for your experiments. Before use, thaw the product at 0°C–4°C, thoroughly mix, and aliquot for use. Avoid more than three freeze-thaw cycles to guarantee consistent performance with each use.
Ordering Information
bioGenous N-2 Serum-Free Supplement is now available for pre-order! Each 10mL bottle contains a highly concentrated supplement, providing robust support for your cell and organoid cultures. We also offer comprehensive technical support and experimental consultation services to assist you in resolving any issues during use.、
Conclusion
bioGenous newly launched N-2 Serum-Free Supplement holds significant application value in cell and organoid culture, demonstrating exceptional performance, particularly in neural cell culture. With its serum-free, chemically defined high-performance formulation, the N-2 Serum-Free Supplement provides a stable and reliable solution for constructing high-quality organoid models. We look forward to collaborating with you to advance progress and development in biological research, disease treatment, and drug development.
