Organoid Single-Cell Functional Genomics (CROP-seq)
By providing services for the establishment of specific organoid models combined with full-process reagents, accompanying genetic manipulation technologies, and single-cell genomics analysis, it is possible to conduct gene function studies under specific organoid model conditions. This approach offers the potential for high-throughput drug screening, tumor drug development, and research into developmental mechanisms.
Service Process
Service Content and Description
Fields
Organoid Single-Cell Omics + CRISPR Screening
Keypoints
Achieving gene function screening at the single-cell level in organoids through CROP-seq technology.
Contents
- Preparation of a lentiviral library of candidate gene sgRNAs;
- Infection of Cas9-expressing organoids, followed by gene editing and application of screening pressure;
- Dissociation of organoids into single cells for single-cell sequencing and analysis.
Advancements
- Compared to traditional cell line screening, gene screening at the organoid level better reflects the true function of genes in vivo;
- Unlike traditional pooled screening methods that only provide a single output metric (cell viability or death), single-cell CROP-seq screening offers comprehensive omics information at the single-cell transcriptome level;
- Single-cell omics screening enables high throughput.
Case Studies
Exploration of Liver Regeneration Regulatory Factors through Single-Cell Functional Genomics of Liver Organoids
Suitable Scenarios
High-Throughput Gene Function Screening Based on Organoids
Related Fields:
Single-Cell Omics, Organoid Models, Development, Regeneration, Tumor Immunology, High-Throughput Screening, Gene Editing, Sequencing
For more details, please contact：
400-600-8315
