Organoid Genetic Manipulation Strategies
Organoid genetic manipulation can utilize the dynamic simulation, high-throughput nature, and short cycle times of organoid models to flexibly perform genetic manipulations, thus enabling the study of gene functions under specific conditions.
Service Process
Service Content and Description
Fields::
Organoid Gene Manipulation
Keypoints:
Achieving efficient gene manipulation in organoids through viral vectors, including overexpression, knockdown, knockout, and stable monoclonal selection.
Contents:
Firstly, the target gene is cloned into the viral core vector. After co-transfecting cells with the auxiliary plasmid to package the virus, it is then co-incubated with the organoids in a planarized manner for 12 hours. Following this, 3D culture is resumed to realize high-throughput gene manipulation in organoid models.
Advancements:
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High Efficiency: Optimized AAV viruses can achieve instantaneous expression efficiencies of over 80%; through lentiviral infection and selection, 100% expression efficiency can be achieved.
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Convenience: After virus packaging is completed, organoid gene manipulation can be achieved within 10 minutes, and gene expression can be observed within 24 hours.
Case Studies
Gene Manipulation and Functional Analysis in Human Liver Organoids Using Adeno-Associated Virus Vector (AAV-DJ)
Journal of Biological Chemistry, 2019, IF=5.2
Suitable Scenarios
Using organoids for specific gene function research, gene studies on cellular lineage conversion, analysis of specific gene functions, and investigation of signaling mechanisms in organoid development.
Related Fields:
Cellular lineage conversion, gene function research, developmental signaling, gene screening, lineage tracing, viral infection
For more details please contact：
400-600-8315
