Fields

Applicable for studying the roles of genes and pathways in tumorigenesis, progression, and metastasis, as well as for investigating patient-specific drug sensitivity and toxicology.

Keypoints

Organoid culture technology, lentiviral transfection technology, xenograft organ transplantation technology based on immunodeficient mice, mRNA sequencing technology, qRT-PCR technology.

Service Process

1. Construct patient-derived tumor organoids.
2. Perform genetic manipulations (e.g., lentiviral transfection to analyze single-gene or multi-gene combinations).
3. Harvest the constructed organoids (approximately 2×10^5 cells in 15 μl of Matrigel) and transplant (inject) them into the corresponding site of 5-7-week-old NSG mice/nude mice.
4. Compare and analyze tumorigenesis and the expression of genes in various pathways.

Advancements

Organoids maintain a high degree of cellular type and functional consistency with their tissue of origin. NSG mice can host a high proportion of normal or cancerous human cells. Therefore, this technology can provide patient-specific preclinical information for tumor patients and offer a convenient and indicative platform.

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"Subcapsular Transplantation of Organoid Liver Forms Human Hepatoblastoma with Lung-Specific Metastasis"

Protein & Cell, 2021, IF=14.9

Specific Roles of Certain Genes in Tumorigenesis and Metastasis; Development of New Drugs for Cancer

Related Fields

Human Hepatoblastoma, Embryonic Development Defects, Pediatric Tumors

For more  details, please contact

400-600-8315