PDOX Construction (Nude Mice, NSG, etc., Immunodeficient Mice)
Transplanting organoids with tissue-specific characteristics into immunodeficient mice such as nude mice or NSG mice enables dynamic simulation of tumor development and embryonic development processes, as well as high-throughput drug screening. The mouse transplantation also achieves in vivo validation.
Service Process
Service Content and Description
Fields
Applicable for studying the roles of genes and pathways in tumorigenesis, progression, and metastasis, as well as for investigating patient-specific drug sensitivity and toxicology.
Keypoints
Organoid culture technology, lentiviral transfection technology, xenograft organ transplantation technology based on immunodeficient mice, mRNA sequencing technology, qRT-PCR technology.
Service Process
- Construct patient-derived tumor organoids.
- Perform genetic manipulations (e.g., lentiviral transfection to analyze single-gene or multi-gene combinations).
- Harvest the constructed organoids (approximately 2×10^5 cells in 15 μl of Matrigel) and transplant (inject) them into the corresponding site of 5-7-week-old NSG mice/nude mice.
- Compare and analyze tumorigenesis and the expression of genes in various pathways.
Advancements
Organoids maintain a high degree of cellular type and functional consistency with their tissue of origin. NSG mice can host a high proportion of normal or cancerous human cells. Therefore, this technology can provide patient-specific preclinical information for tumor patients and offer a convenient and indicative platform.
Case Studies
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"Subcapsular Transplantation of Organoid Liver Forms Human Hepatoblastoma with Lung-Specific Metastasis"
Protein & Cell, 2021, IF=14.9
Suitable Scenarios
Specific Roles of Certain Genes in Tumorigenesis and Metastasis; Development of New Drugs for Cancer
Related Fields
Human Hepatoblastoma, Embryonic Development Defects, Pediatric Tumors
For more details, please contact:
400-600-8315
