New Product Launch | bioGenous™ NK Cell Expansion Medium (Serum-Free) Serum-Free NK Cell Culture Medium
Publication Date：2024-06-28
Foreword
Natural killer cells (NK cells) are crucial immune cells in the body, playing roles not only in antitumor activity, antiviral defense, and immune regulation, but also participating in hypersensitivity reactions and the development of autoimmune diseases under certain conditions. They are capable of recognizing and killing target cells.
Traditional NK cell culture media typically contain heterologous serum components, such as bovine serum or other animal-derived ingredients. This introduces risks of contamination with exogenous viruses and pathogenic factors during cell culture, leading to poor reproducibility of products and experimental results. Developing serum-free media can reduce variables in the experimental process, minimize batch-to-batch variations in media, enhance experimental reproducibility and standardization, while simultaneously avoiding ethical concerns associated with animal serum use. This aligns with the sustainability and ethical trends in modern biotechnology.
Product Introduction
bioGenous™ NK Cell Expansion Medium (Serum-Free) is a specially formulated medium designed for the efficient in vitro expansion of human NK cells. The entire culture process requires no serum and contains no heterologous components. This medium contains all essential nutrients required for NK cell growth, supporting both the expansion and differentiation of NK cells derived from peripheral blood mononuclear cells (PBMCs). It is also suitable for the reactivation culture of NK cells. The expanded NK cells can support various in vitro studies, including drug activity assays and co-culture experiments with organoids. Consequently, this medium serves as an ideal assistant for NK cell-related research.
Product Features
Stable and Safe
Free from heterologous components; clearly defined chemical composition
Serum-free, pure factor reagent kit
Manufactured and managed under GMP standards
Broadly Applicable
Suitable for inducing NK cells from peripheral blood and umbilical cord blood
Enables highly efficient in vitro expansion of NK cells
High cost-effectiveness and ease of use, suitable for industrial-scale production
Product Data Presentation
PBMC In Vitro Proliferation:
Figure 1 Comparison of in vitro growth states of PBMCs isolated from different patients
Initial cell count: 10,000 cells per well. Perform a 50% medium change every two days, replenishing with fresh medium. After 15 days of culture, distinct cell clusters become visible.
Figure 2Comparison of in vitro proliferation of PBMCs isolated from different patients as detected by CFSE staining
In vitro proliferation of NK cells in PBMCs
Figure 3NK Cell Proliferation in PBMCs: A) Flow Cytometry CD3-CD56+ Density Plot; B) Line Graph of NK Cell Proportion and Line Graph of NK Cell ProliferationFold Increase
Evaluation of NK Cell Proliferation in Vitro Following Sorting
Figure 4 NK Cell Proliferation: A) Flow cytometry screening of CD3-CD56+ density plot; B) Bar chart of NK cell growthfold increase
