bioGenous™ Mouse Intestinal Organoid Prime Kit is a defined, serum‑free, 2‑component culture system. Compared with the previous 3‑component formulation, it greatly simplifies preparation procedures, improves stability and traceability, and enables efficient establishment and long‑term maintenance of mouse small intestinal organoids. Through systematic optimization of the ratios of key factors and supportive environment in the culture system, the Prime version significantly enhances operational convenience, crypt budding efficiency, organoid formation speed, passaging expansion stability, and inter‑batch reproducibility. It is suitable for intestinal stem cell research, epithelial regeneration studies, tumor modeling, gene editing, drug discovery and other related applications.