产品列表

联系方式

400-600-8315

info@biogenous.cn

浙江省杭州市萧山区宁围街道传化科创大厦2幢6层

Recombinant Human R-spondin1

目录号
861-RS1
产品规格
{{item.Title}}
现价:¥{{price}}
原价:¥{{oldPrice}}
产品介绍

Background:

R-Spondin1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Postnatally, R-Spondin1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate. R-Spondin1 regulates Wnt/β-catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt coreceptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization. Recombinant Human R-Spondin1 is a 27.3 kDa protein consisting of 233 amino acid residues. Due to glycosylation, RSpondin1 migrates at an apparent molecular weight of approximately 41.0 kDa by SDS-PAGE analysis under reducing conditions.

 


技术参数

Source:

Chinese Hamster Ovary cell line

Protein Construction:

A DNA sequence encoding the amino acids (Ala31-Ser263) of human Rspo-1 (Accession Number: Q2MKA7) was expressed.

Synonyms:

Roof plate-specific spondin-1, RSPO1

Purity:

≥ 95%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.

Biological Activity:

Measured by its ability to induce activation of β-catenin response in a Top-flash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.5 μg/ml in the presence of 5 ng/ml recombinant mouse Wnt3a.

Endotoxin Level:

<0.10 EU per 1 μg of the protein by the LAL method

Calculated Molecular Weight:

27.3 kDa

SDS-PAGE:

41.0 kDa, reducing condition

Formulation:

The product is Lyophilized from a 0.22μm filtered solution in PBS.

Shipping:

The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.

Reconstitution:

Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Stability & Storage:

24 months, -20 to -70 °C, under powder state; 12 months, -20 to -70 °C, under sterile conditions after reconstitution; 2 month, 2 to 8 °C under sterile conditions after reconstitution; avoid repeated freeze-thaw cycles.

References:

  1. Kim, K.A., et al., R-Spondin proteins: a novel link to beta-catenin activation. Cell Cycle, 2006. 5(1)
  2. Kamata, T., et al., R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta, 2004. 1676(1)
  3. Hao, H.X., et al., ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature, 2012. 485(7397) 
分享到微信