R-Spondin1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Postnatally, R-Spondin1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate. R-Spondin1 regulates Wnt/β-catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt coreceptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization. Recombinant Human R-Spondin1 is a 27.3 kDa protein consisting of 233 amino acid residues. Due to glycosylation, RSpondin1 migrates at an apparent molecular weight of approximately 41.0 kDa by SDS-PAGE analysis under reducing conditions.
Chinese Hamster Ovary cell line
A DNA sequence encoding the amino acids (Ala31-Ser263) of human Rspo-1 (Accession Number: Q2MKA7) was expressed.
Roof plate-specific spondin-1, RSPO1
≥ 95%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.
Measured by its ability to induce activation of β-catenin response in a Top-flash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.5 μg/ml in the presence of 5 ng/ml recombinant mouse Wnt3a.
<0.10 EU per 1 μg of the protein by the LAL method
Calculated Molecular Weight:
41.0 kDa, reducing condition
The product is Lyophilized from a 0.22μm filtered solution in PBS.
The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.
Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Stability & Storage:
24 months, -20 to -70 °C, under powder state; 12 months, -20 to -70 °C, under sterile conditions after reconstitution; 2 month, 2 to 8 °C under sterile conditions after reconstitution; avoid repeated freeze-thaw cycles.