Basement membranes are thin continuous layers of specialized extracellular matrix that form the interface on which epithelial, endothelial, or neuronal cells grow; basement membranes also support muscle and Schwann cells of the peripheral nerves as they are found surrounding these cells. bioGenous™ EasyGel 3D ECM is a soluble form of basement membrane extracted and purified from the Engelbreth-Holm-Swarm (EHS) tumor, and the major constituents of the bioGenous™ EasyGel 3D ECM include laminin, collagen IV, entactin/nidogen, and heparin sulfate proteoglycan.
bioGenous™ EasyGel 3D ECM is a reduced growth factor hydrogel specifically optimized for rapid organoid construction, which allows the complete control of the organoid culture microenvironment to allow for more consistent cell growth and reproducible experiments; it supports the growth and maintenance of human embryonic stem cells (hESCs), tissue-derived stem cells, and for the in vivo study of both healthy and tumor organoids derived from various organs, and it is compatible with all culture media and gels at temperatures above 10°C to form a reconstituted basement membrane. Notably, bioGenous™ EasyGel 3D ECM is highly suitable for PDX-derived organoid (PDXO) models, providing an optimal scaffold for tumor cells isolated from patient-derived xenografts to maintain their original histological architecture, phenotypic traits, and genetic stability.
