bioGenous™OrganoQC Anti-Fungal Supplement (1000X) is a mixed antimicrobial supplement specifically designed for organoid culture, with a broad antimicrobial spectrum covering fungi, yeasts, and bacteria. During organoid establishment, insufficient washing of biopsy or surgically resected tissues—especially those derived from the gastrointestinal tract, biliary tract, respiratory tract, and cervix—may introduce yeasts or environmental fungi. In addition, organoid culture procedures involving basement membrane matrices, culture media handling, frequent plate opening, and air exposure increase the risk of introducing fungal spores. Once contamination occurs, organoid cultures are typically unusable. Therefore, preventive antimicrobial supplementation is strongly recommended, particularly when working with valuable primary organoid samples.

Amphotericin B is a polyene antifungal agent that irreversibly binds to sterols (primarily ergosterol) in fungal cell membranes, forming transmembrane channels that disrupt membrane integrity. This leads to leakage of intracellular small molecules such as monovalent ions (K⁺, Na⁺, Cl⁻), nucleotides, and amino acids, ultimately impairing normal fungal metabolism and causing cell death. Therefore, Amphotericin B is widely used to suppress fungal and yeast contamination. Gentamicin is an aminoglycoside antibiotic effective against a wide range of Gram-positive and Gram-negative bacteria. It functions by binding to the bacterial 30S ribosomal subunit, thereby inhibiting protein synthesis and leading to bacterial cell death. In addition, gentamicin also exhibits inhibitory activity against certain mycoplasma contaminants.

This product contained 250 μg/mL Amphotericin B and 50 mg/mL Gentamicin. The recommended working concentrations in cell culture medium are 50 μg/mL for gentamicin and 0.25 μg/mL for Amphotericin B, corresponding to a 1:1000 dilution of this supplement. This product is sterile-filtered and can be directly added to organoid culture media. It is also suitable for tissue washing steps during primary organoid establishment.