bioGenous™ Human Hepatocyte Organoid Differentiation Kit is a serum-free culture medium specifically designed to induce the differentiation of human hepatocyte organoids derived from adult hepatocytes. This kit is intended to be used in combination with the bioGenous™ Human Hepatocyte Organoid Expansion Kit (Cat. K2513-HHE). This kit promotes the maturation of hepatocyte organoids toward a functionally differentiated state, enabling the formation of hepatocyte-like cells with metabolic activity. Organoids differentiated using this kit express key mature hepatic markers such as CYP3A4, CYP1A2 and CYP2E1, reflecting the establishment of functional epithelial architecture and polarized hepatic organization. The differentiated organoids exhibit spatial zonation markers (e.g., CV-region CYP2E1 and PV-region GLUT1), recapitulating the metabolic heterogeneity of liver lobules in vivo. This kit supports controlled and reproducible differentiation in a defined serum-free system, making it well-suited for applications in drug metabolism studies, liver disease modeling, and advanced hepatic function assays. bioGenous™ Human Hepatocyte Organoid Differentiation Kit is an ideal tool for researchers seeking to generate mature liver tissue-like organoids for in vitro functional analysis and translational research.