bioGenous™ Human Hepatocyte Organoid Differentiation Kit is a chemically defined, serum-free system engineered to drive the functional maturation of adult hepatocyte-derived organoids. This kit is intended to be used in combination with the bioGenous™ Human Hepatocyte Organoid Expansion Kit (Cat. K2513-HHE). This kit effectively recapitulates the complex metabolic heterogeneity of the liver lobule in vivo. By inducing robust spatial zonation, the kit promotes the differential expression of key metabolic markers, specifically localizing central vein (CV)-like regions (CYP2E1+) and periportal (PV)-like zones (GLUT1+). This high-fidelity model yields mature organoids with superior metabolic activity, providing a highly reproducible and standardized tool for drug metabolism studies, liver disease modeling, and advanced translational research.