产品列表

联系方式

400-600-8315

info@biogenous.cn

浙江省杭州市萧山区宁围街道传化科创大厦2幢6层

Recombinant Human FGF10

目录号
816-FGF
产品规格
{{item.Title}}
现价:¥{{price}}
原价:¥{{oldPrice}}
产品介绍

Background:

FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) [1]. The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung [2, 3]. Overlapping expression patterns and activities with FGF-3, -7, and -8 suggest at least a partial redundancy in FGF‑10 biology. FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer [4] .

 


技术参数

Source:

E.coli

Protein Construction:

A DNA sequence encoding the amino acids (Gln38-Ser208) of human FGF10 (Accession Number: O15520) was expressed.

Synonyms:

Fibroblast growth factor 10; Keratinocyte growth factor 2; KGF2; KGF-2. Purity:

≥ 95%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.

Biological Activity:

Measured in a cell proliferation assay using human liver ductal organoids. The ED50 for this effect is 20-100 ng/ml.

Endotoxin Level:

<0.10 EU per 1 μg of the protein by the LAL method

Calculated Molecular Weight:

19.5 kDa

SDS-PAGE:

25 kDa, reducing condition

Formulation:

The product is Lyophilized from a 0.22μm filtered solution in PBS.

Shipping:

The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.

Reconstitution:

Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Stability & Storage:

24 months, -20 to -70 °C, under powder state; 12 months, -20 to -70 °C, under sterile conditions after reconstitution; 2 month, 2 to 8 °C under sterile conditions after reconstitution; avoid repeated freeze-thaw cycles.

References:

  1. Zhang, X., et al., Receptor specificity of the fibroblast growth factor family. The complete mammalian FGF family. J Biol Chem, 2006. 281(23): p. 15694-700.
  2. Bellusci, S., et al., Fibroblast growth factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung. Development, 1997. 124(23): p. 4867-78.
  3. Weaver, M., N.R. Dunn, and B.L. Hogan, Bmp4 and Fgf10 play opposing roles during lung bud morphogenesis. Development, 2000. 127(12): p. 2695-704.
  4. Nomura, S., et al., FGF10/FGFR2 signal induces cell migration and invasion in pancreatic cancer. Br J Cancer, 2008. 99(2): p. 305-13.
分享到微信