bioGenous™ Human Colonic Organoid Kit is a serum-free culture medium for human colonic organoids derived from adult intestinal stem cells. Self-renewal of colonic epithelium is driven by the proliferation of stem cells and their progenitors located in the crypts. Human colonic organoids grown in the Human Colonic Organoid Expansion Medium overwhelmingly consisted of LGR5+ stem cells, cycling transit amplifying (TA) cells, early enterocytes and a small number of goblet cells. Organoids grown in Human Colonic Organoid Maintenance Medium contain LGR5+ stem cells, TA cells, early and mature enterocytes, goblet cells, M cells and enteroendocrine cells, as well as a low number of tuft cells. Human colonic organoids display hallmarks of the colonic epithelium in terms of architecture and cell type composition, therefore hold great promise for studies of the development and physiological characteristics of the human colon, as well as the mechanisms of colonic-related biological changes, human colonic organoids may also have applications in basic biological research through in vitro initial culture of the colonic epithelium.